Journal: Journal of Materials Science. Materials in Medicine

Article Title: SDF-1α gene-activated collagen scaffold enhances provasculogenic response in a coculture of human endothelial cells with human adipose-derived stromal cells

doi: 10.1007/s10856-021-06499-6

Figure Lengend Snippet: Effect of PEI–pSDF-1α transfection on HUVECs and its impact on ADSCs. A PEI–pSDF-1α transfected HUVECs exhibited significantly enhanced survivability response compared to those transfected nontherapeutic PEI–pLuc polyplex. B Transfection with PEI–pSDF-1α induced transient production of the target protein over a period of 2 weeks. C Phase-contrast microscopy images of morphological changes in HUVECs and its coculture with ADSCs at day 14. i Untransfected HUVECs maintained its cobblestone-like morphology. ii Transfected HUVECs appeared more polarized. iii Addition of ADSCs to untransfected HUVECs resulted in the formation of interconnected cellular network within the monolayer assembly. iv Elongated, pseudo three-dimensional dense cellular clusters were formed in transfected coculture group. Data are plotted as mean ± standard deviation ( n = 6)

Article Snippet: Human umbilical vein endothelial cells (HUVECs) and ADSCs were obtained from Cell Applications, Inc. and iXCells Biotechnologies, USA, respectively.

Techniques: Transfection, Microscopy, Standard Deviation

Journal: Journal of Materials Science. Materials in Medicine

Article Title: SDF-1α gene-activated collagen scaffold enhances provasculogenic response in a coculture of human endothelial cells with human adipose-derived stromal cells

doi: 10.1007/s10856-021-06499-6

Figure Lengend Snippet: Temporal regulation of soluble VE-cadherin from endothelialized gene-free scaffold and SDF-1α GAS. SDF-1α GAS strongly affects the vascular growth of endothelial cells by suppressing the release of soluble VE-cadherin. Coculturing with ADSCs offers further control on the release of soluble VE-cadherin from endothelial cells. HUVECs on SDF-1α GAS demonstrated significant reduction in the levels of soluble VE-cadherin at days 7 ( p < 0.05) and 10 ( p < 0.0005) relative to HUVECs on gene-free scaffold. Coculture on SDF-1α GAS strongly attenuated the release of VE-cadherin at all time points. Data are presented as mean ± standard deviation. One-way ANOVA was used to deduce statistical significance. *, **, ***, and **** indicate statistical significance of p < 0.05, p < 0.01, p < 0.005, and p < 0.0005, respectively

Article Snippet: Human umbilical vein endothelial cells (HUVECs) and ADSCs were obtained from Cell Applications, Inc. and iXCells Biotechnologies, USA, respectively.

Techniques: Control, Standard Deviation

Journal: Journal of Materials Science. Materials in Medicine

Article Title: SDF-1α gene-activated collagen scaffold enhances provasculogenic response in a coculture of human endothelial cells with human adipose-derived stromal cells

doi: 10.1007/s10856-021-06499-6

Figure Lengend Snippet: Gene expression analysis of HUVECs and its coculture on gene-free scaffolds and SDF-1α GAS. SDF-1α GAS significantly increased the expression of mRNAs for SDF-1α and its cognate receptor CXCR4 in HUVECs. Coculture with ADSCs significantly elevated the expression of downstream effector genes of SDF-1α—CXCR4 and eNOS than the HUVECs on SDF-1α GAS. Data are plotted as mean ± standard deviation ( n = 3). *, **, and *** indicate statistical significance of p < 0.05, p < 0.01, and p < 0.005, respectively

Article Snippet: Human umbilical vein endothelial cells (HUVECs) and ADSCs were obtained from Cell Applications, Inc. and iXCells Biotechnologies, USA, respectively.

Techniques: Expressing, Standard Deviation

Journal: Journal of Materials Science. Materials in Medicine

Article Title: SDF-1α gene-activated collagen scaffold enhances provasculogenic response in a coculture of human endothelial cells with human adipose-derived stromal cells

doi: 10.1007/s10856-021-06499-6

Figure Lengend Snippet: Visualization of endothelial anastomosis and SDF-1α expression. A All the cultures showed strong immunoreactivity to CD31 and exhibited endothelial morphogenesis representative of capillary-like network. B HUVECs on the gene-free scaffold expressed the lowest amount of SDF-1α proteins. C Magnified images of the endothelial network showing the differences in spatial distribution of SDF-1α between the groups. D Quantitative representation of SDF-1α protein expression showing an increasing trend toward the coculture group. Data are presented as mean ± standard deviation. * indicates statistical significance of p < 0.05

Article Snippet: Human umbilical vein endothelial cells (HUVECs) and ADSCs were obtained from Cell Applications, Inc. and iXCells Biotechnologies, USA, respectively.

Techniques: Expressing, Standard Deviation

Journal: Materials Today Bio

Article Title: Redirecting the route: Monocyte-mediated delivery of oHSV-1 across a human BBB-on-chip model

doi: 10.1016/j.mtbio.2025.102458

Figure Lengend Snippet: Microfluidic BBB-on-chip design and validation. A. Schematic representation of the BBB-on-chip, seeded with endothelial cells on the blood side and pericytes and astrocytes on the brain side, equipped with three microwells for 3D GBM spheroid culture (GBM spheroids area). B. Image of the assembled BBB-on-chip showing the upper channel (yellow) mimicking the vascular compartment and main perfusion conduit, and the lower channels (red) leading to the tumor compartment (scale bar = 2 cm). C. Transendothelial electrical resistance (TEER) measurements recorded daily across the membrane between the upper and lower channels and supporting the BBB. D. Permeability coefficients calculated from the diffusion of 40, 70, and 150 kDa FITC-dextrans through the assembled BBB-on-chip in the following conditions: i. No cells (control, membrane without cells), ii. Blood side (membrane + HUVECs endothelial monolayer), and iii. BBB complete (membrane with the three-culture of HUVECs on the blood side and pericytes and astrocytes on the brain side). E . IF staining of the BBB-on-chip model on day 7. On the blood side compartment , endothelial tight junction protein ZO-1 (green) and the endothelial marker CD31 (green) are expressed, confirming the formation of a continuous endothelial layer. On the brain side , astrocytes are identified by GFAP expression (magenta), indicating appropriate localization. DAPI (blue) highlights cell nuclei in both compartments. Scale bar = 50 μm. Values are expressed as median±SEM from at least 3 independent experiments. ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001 vs control. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The assembled BBB-on-chip devices were bound to glass coverslips via further plasma treatment and sterilized with 70 % ethanol followed by UV exposure for 60 min. BBB-on-chip cells seeding and culturing: Human umbilical vein endothelial cells (HUVECs), brain vascular pericytes (hBVPs), and immortalized astrocytes (hAs) were obtained from Innoprot (Derio, Spain).

Techniques: Biomarker Discovery, Membrane, Permeability, Diffusion-based Assay, Control, Staining, Marker, Expressing